Nuance EX
Multispectral microscopy from VIS to NIR
The Nuance EX multispectral imaging system allows samples to be imaged along a very wide multispectral range from 450 to 950 nm. Most chromagens and fluorophores emit within this range, allowing for in vivo or ex vivo imaging of near-infrared (NIR) fluorophores. The Nuance EX system can be used for both chromogenic or fluorescence-based imaging; however, the Nuance FX may be better suited for certain chromogenic applications. The spectral bandwidth is adjustable for sensitivity or throughput with no moving parts, and has been optimized for NIR applications.
Nuance software incorporates powerful unmixing and analysis algorithms, as well as easy-to-use tools for building your own spectral libraries for repetitive use. View software clips to learn more about how Nuance captures, unmixes and quantitates. Nuance is now compatible with Molecular Devices’ MetaMorph™.
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Figure: Screenshot of the Nuance software showing a lymph node section courtesy Raul Braylan, University of Florida.
Removal of autofluorescence with increased contrast and legibility. Sample: Tonsil (FFPE, 10X) stained for kappa (Qdot 605) and lambda (Qdot 655). Nuance composite image shows kappa and lambda signals with autofluorescence removed.
Sample courtesy Ventana Medical Systems.
