Maestro™
The standard for all-optical in vivo imaging
Award-winning Maestro systems enable multiplexed in vivo fluorescence imaging of small animals with unprecedented sensitivity. Maestro offers users the capability of removing autofluorescence emitted from images of skin and other tissues to reveal otherwise hard-to-detect labeled targets. The dramatic improvement in signal-to-noise can increase sensitivity up to several hundred-fold, enabling much smaller or fainter signals from biological targets to be detected earlier and accurately measured. The increased sensitivity over standard imaging techniques provided by Maestro’s high-quality spectral imaging technology enable experiments and models which simply cannot be done with any other system.
Upper mouse has a tumor in the left groin, revealed by an antibody bound to Alexa Fluor 680. The rest of the shaved area is non-specific signal. Lower mouse is a negative control mouse.
Sample courtesy UC-Davis Molecular Imaging Workshop
Note: Alexa Fluor® 680 is a registered trademark of the Invitrogen Corporation.
The Maestro system comes in two models:
| New Maestro EX | Maestro 2 | |
|---|---|---|
| Filter Selection | Manual | Automated |
| Stage Adjustment | Manual | Automated |
| Light-Tight Chamber |  |
|
| Wavelength Range | 500 – 950 nm | 500 – 950 nm |
| Bandwidth Selections | 20 and 40 nm | 20 and 40 nm |
| Min Wavelength Spacing | 2 nm | 2 nm |
| Fluorescence Sensitivity* | 300x | 300x |
| CCD / Cooling | Sony ICX285 / 8°C | Sony ICX285 / 0°C |
| Spatial Resolution | 25 micron/pixel | 25 micron/pixel |
| Max Field-of-View | Up to 3 mice | Up to 3 mice |
| Typical Exposure Time | 2 – 5 sec | 2 – 5 sec |
| Max Exposure Time | 10 min | 20 min |
| Anatomic Organ Maps and Kinetic Imaging | Available with DyCE | Available with DyCE |
| *Maximum sensitivity over standard monochrome imaging methods. | ||
The Maestro system can acquire data from up to three mice simultaneously with up to 25 micron/pixel resolution in only a few seconds, and can detect, measure and track multiple fluorophores in vivo. CRi’s Maestro has won the prestigious R&D Magazine’s R&D 100 award, considered the “Oscars of Innovation”. In combination with CRi’s Nuance system, our solutions can provide resolution ranging from multiple mice or a rat to the subcellular level, enabling accurate and consistent ex vivo validation of in vivo findings.
Maestro users can also benefit from CRi's newly released Dynamic Contrast Enhancement solution (DyCE™):
CRi's new all-optical anatomic imaging solution that generates 3D organ maps and anatomical target co-localization. The Maestro system can acquire data from up to three mice simultaneously down to 25 micron/pixel resolution in only a few seconds, and can detect, measure and track multiple fluorophores in vivo. CRi's Maestro has won the prestigious R&D Magazine's R&D 100 award, considered the "Oscars" of invention. In combination with CRi's Nuance system, it can provide resolution ranging from multiple mice or a rat to the subcellular level, enabling accurate and consistent ex vivo validation of in vivo findings.
