Fluorescence Microscopy
Multi-label microscopy without cross-talk from overlapping labels or autofluorescence
Fluorescence microscopy is a critical tool for academic and pharmaceutical research, pathology, and clinical medicine. However, there is an ever increasing need for multiplexed fluorescent marker studies, as the complexity of the biological questions being asked grows. Until now, there have been no practical, fast, or economical means to assess multiple protein and pathway configurations in tissue sections, on a cell-by-cell basis. CRi’s Nuance system enables quantitative multiplexing of multiple fluorescent molecular markers in intact tissue sections at an affordable price and in a high-throughput format. CRi’s Nuance system can also effectively eliminate the confounding effects of autofluorescence, revealing otherwise invisible molecular targets, and improving contrast and quantitation accuracy.
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Image 1: Four Alexa fluor dyes in mouse intestine. (Molecular Probes)
Image 2: Five QDots in lymph node germinal center. (S Pittaluga, NCI)
Image 3: Triple-labeled liver tissue. (L Komuves, Genentech)
Multiplexed imaging of quantum-dot-stained tissue. Breast cancer (FFPE, 20X) stained for estrogen receptor (ER, Qdot 655 nm) and progesterone receptor (PR, Qdot 585 nm). Nuclei counterstained with DAPI, and tissue autofluorescence detected spectrally. Separate planes for each signal are shown, and at the second Nuance composite image displayed in Brightfield Display Mode (BDM).
